Journal: Journal of Visualized Experiments : JoVE

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

doi: 10.3791/3791

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Article Snippet: Name of the reagent s/equipments Company Catalogue number Non contact microarrayer BioDot Inc sciFLEXARRAYER 384 microplate Fisher 14-230-243 FoodSaver FoodSaver V3835 Ultrathin nitrocellulose coate microarray slides Gentel PATH Slide Imprinter (optional) The Gel Company WSP60-1 Shaker Fisher 15-453-211 Centrifuge Eppendorf 5804 000.013 Slide washing basin/Slide Staining Dish with Removable Rack Fisher 08-812 Slide incubation chamber/microscope slide box Fisher 03-448-5 Brij 35, 30 w/v% solution in water Acros Organics AC32958-0025 Tween-20 Fisher P337-100 Sodium Periodate (NaIO 4 ) Sigma 311448 L-Glutamic acid γ-hydrazide Sigma G-7257 Sodium Acetate Anhydrous (CH 3 COONa) Sigma S2889 Bovine Serum Albumin (BSA) Lampire Biological Labs 7500804 Phosphate Buffer Saline (PBS) (10X) Denville Scientific CP4390-48 Dylight 549 conjugated NeutrAvidin Thermo 22837 Protease Inhibitor Cocktail Tablets Roche 4693159001 ChromPure Human IgG, Fc fragment Jackson Immunoresearch 009-000-008 ChromPure Human IgG, whole molecule Jackson Immunoresearch 009-000-003 ChromPure Mouse IgG, whole molecule Jackson Immunoresearch 015-000-003 ChromPure Mouse IgG, Fc fragment Jackson Immunoresearch 015-000-008 ChromPure Rabbit IgG, whole molecule Jackson Immunoresearch 011-000-003 ChromPure Donkey IgG, whole molecule Jackson Immunoresearch 017-000-003 Microarray Scanner Tecan LS Reloaded Open in a separate window Table 2.

Techniques: Microarray, Staining, Incubation, Saline, Protease Inhibitor